| Formulation |
TOP1 is supplied at a concentration of 1mg/1ml in 16mM HEPES buffer pH-7.5, 400mM NaCl and 20% glycerol. |
| Purity |
Greater than 70% as determined by SDS-PAGE. |
| Applications |
Western blot with Scleroderma positive patient sera. |
| Description |
Bovine DNA Topoisomerase-I purified from bovine tissues by proprietary chromatographic techniques. |
| Protein Background |
TOP1 is an important nuclear enzyme that interconverts supercoiled DNA to the necessary topological conformations for standard DNA replication and transcription. TOP1 is the target antigen for TOP1 autoantibodies. TOP1 antibodies are a specific marker in scleroderma patients (specificity 98-100%) and are related with the existence of diffuse skin involvement and pulmonary fibrosis. In human tissues top1 enzyme is primarily synthesized as a protein with a molecular weight of 100-kDa. Most of this precursor is then proteolytically processed to a 70-kDa size, from which the TOP1 antigen has derived its name. |
| Expression host |
Bovine tissues. |
| Synonyms |
DNA topoisomerase 1, EC 5.99.1.2, DNA topoisomerase I, TOP1, Scl-70. |
| Storage Conditions |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Immunological functions |
1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test (checkerboard analysis of positive/negative sera panels) and immunodot test with positive/negative sera panels. |
| Coating concentration |
0.5-0.8 ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups. |
