| Reconstitution |
1mg/ml in PBS (after reconstitution). |
| Formulation |
TNFRSF10C protein solution (1mg/ml) containing Phosphate buffered saline (pH7.4) and 10% glycerol. |
| Purity |
Greater than 90.0% as determined by SDS-PAGE. |
| Description |
TNFRSF10C Human Recombinant produced in E. coli is a single, non-glycosylated polypeptide chain containing 234 amino acids (26-236 a.a) and having a molecular mass of 24.6 kDa.TNFRSF10C is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
TRAIL Receptor-3, also known as TNFRSF10C belongs to the TNF-receptor super family. TNFRSF10C contains an extracellular TRAIL-binding domain as well as a transmembrane domain, however no cytoplasmic death domain. Furthermore, TNFRSF10C is not capable of inducing apoptosis, and is considered to act as an antagonistic receptor which protects cells from TRAIL-induced apoptosis. |
| Expression host |
Escherichia Coli. |
| Synonyms |
Tumor Necrosis Factor Receptor Superfamily, Member 10c, Decoy Without An , Intracellular Domain, TRAILR3, DCR1, TRID, TNF-Related Apoptosis-Inducing Ligand Receptor, Antagonist Decoy Receptor For TRAIL/Apo-2L, Decoy TRAIL Receptor Without Death Domain, Lymphocyte Inhibitor Of TRAIL, Decoy Receptor,TRAIL-R, LIT,Tumor Necrosis Factor Receptor Superfamily Member 10C, TRAIL Receptor Without an Intracellular Domain, Cytotoxic TRAIL Receptor, TRAIL Receptor, CD263 Antigen, DCR1-TNFR, CD263. |
| Reagent Appearance |
Sterile Filtered clear solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSATTARQE EVPQQTVAPQ QQRHSFKGEE CPAGSHRSEH TGACNPCTEG VDYTNASNNE PSCFPCTVCK SDQKHKSSCT MTRDTVCQCK EGTFRNENSP EMCRKCSRCP SGEVQVSNCT SWDDIQCVEE FGANATVETP AAEETMNTSP GTPAPAAEET MNTSPGTPAP AAEETMTTSP GTPAPAAEET MTTSPGTPAP AAEETMTTSP GTPA. |
| Antibody Properties |
Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. |
| Titer |
Antibody is shipped lyophilized at ambient temperature. |
| Protein content |
For intra-cellular staining, use 5-10ul/1,000,000 cells. Wash twice with PBS/Azide before adding the appropriate amount of secondary fluorescent reagent. |
| Other Available Forms |
Vimentin antibody will recognize most mammalian Vimentin except mouse and rat. |
| Storage/ Stability |
This antibody is also available conjugated to biotin and FITC. |
