TK2 Human

Data sheet

Formulation

TK2 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer, pH8.0, 30% glycerol, 2mM DTT and 200mM NaCl.

Purity

Greater than 85% as determined by SDS-PAGE.

Description

TK2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 257 amino acids (34-265 a.a) and having a molecular mass of 30.2kDa.TK2 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromat

Protein Background

Thymidine kinase 2 mitochondrial (TK2) is a member of the DCK/DGK family. TK2 is an enzyme, a phosphotransferase (a kinase): 2'-deoxythymidine kinase, ATP-thymidine 5'-phosphotransferase. Thymidine kinase is found in most living cells. Thymidine kinase is

Expression host

E.coli.

Synonyms

Thymidine kinase 2 mitochondrial, Mt-TK, TK2, MTTK, MTDPS2.

Reagent Appearance

Sterile Filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSHMVQRRA WPPDKEQEKE KKSVICVEGN IASGKTTCLE FFSNATDVEV LTEPVSKWRN VRGHNPLGLM YHDASRWGLT LQTYVQLTML DRHTRPQVSS VRLMERSIHS ARYIFVENLY RSGKMPEVDY VVLSEWFDWI LRNMDVSVDL IVYLRTNPET CYQRLKKRCR EEEKVIPLEY LEAIHHLHEE WLIKGSLFPM AAPVLVIEAD HH