TGFA Human

Data sheet

Formulation

Lyophilized from a 0.2um filtered solution in Acetonitrile and TFA.

Solubility

It is recommended to reconstitute the lyophilized TGFA in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 97.0% as determined by SDS-PAGE.

Description

TGFA Human Recombinant (40-89) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 50 amino acids and having a molecular mass of 6kDa.The TGFA is purified by proprietary chromatographic techniques.

Protein Background

Transforming Growth Factor-Alpha (TGF-alpha) belongs to the EGF family of cytokines. TGFA soluble form is discharged from the membrane by proteolytic cleavage. Membrane-bound proTGF-alpha is biologically active and has a role in cell-cell adhesion or in t

Expression host

Escherichia Coli.

Synonyms

Transforming Growth Factor Alpha, Protransforming Growth Factor Alpha, TGF-Alpha, TGFA.

Stability

Lyophilized TGFA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TGFA should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a ca

Amino acid sequence

VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA.

Physical Appearence

Sterile Filtered White lyophilized (freeze-dried) powder.

Biological Activity

The ED50, as measured in a proliferation assay using mouse Balb/3T3 cells, is less than 0.4ng/ml.