| Formulation |
Lyophilized from a 0.2um filtered solution in Acetonitrile and TFA. |
| Solubility |
It is recommended to reconstitute the lyophilized TGFA in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Purity |
Greater than 97.0% as determined by SDS-PAGE. |
| Description |
TGFA Human Recombinant (40-89) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 50 amino acids and having a molecular mass of 6kDa.The TGFA is purified by proprietary chromatographic techniques. |
| Protein Background |
Transforming Growth Factor-Alpha (TGF-alpha) belongs to the EGF family of cytokines. TGFA soluble form is discharged from the membrane by proteolytic cleavage. Membrane-bound proTGF-alpha is biologically active and has a role in cell-cell adhesion or in the stimulation of adjacent cells. TGFA expression is common in transformed cells. Additionally, TGFA is expressed in normal tissues during embryogenesis and in adult cells/tissues, including the pituitary, keratinocytes, and macrophages. |
| Expression host |
Escherichia Coli. |
| Synonyms |
Transforming Growth Factor Alpha, Protransforming Growth Factor Alpha, TGF-Alpha, TGFA. |
| Stability |
Lyophilized TGFA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TGFA should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Amino acid sequence |
VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA. |
| Physical Appearence |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Biological Activity |
The ED50, as measured in a proliferation assay using mouse Balb/3T3 cells, is less than 0.4ng/ml. |
