| Formulation |
The TBCB solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM Nacl and 20% glycerol. |
| Purity |
Greater than 90% as determined by SDS-PAGE. |
| Description |
TBCB Human Recombinant produced in E. coli is a single polypeptide chain containing 268 amino acids (1-244) and having a molecular mass of 29.9 kDa.TBCB is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
TBCB is a member of the Microtubules family. Biosynthesis of functional microtubules include the involvement of a number of chaperones, termed Tubulin folding cofactors A (TBCA), B (TBCB), C (TBCC), D (TBCD) and E (TBCE), which operate on folding intermediates downstream of the cytosolic chaperon, known as TCP. The 244 amino acid cytoplasmic protein TBCB has one CAP-Gly domain and is universally expressed. TBCB takes part in the regulation of tubulin heterodimer dissociation and operates as a negative regulator of axonal growth. |
| Expression host |
E.coli. |
| Synonyms |
Tubulin folding cofactor B, Cytoskeleton-associated protein CKAPI, cytoskeleton associated protein 1, CKAP1, CG22, Tubulin-specific chaperone B. |
| Reagent Appearance |
Sterile Filtered colorless solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSHMEVTGV SAPTVTVFIS SSLNTFRSEK RYSRSLTIAE FKCKLELLVG SPASCMELEL YGVDDKFYSK LDQEDALLGS YPVDDGCRIH VIDHSGARLG EYEDVSRVEK YTISQEAYDQ RQDTVRSFLK RSKLGRYNEE ERAQQEAEAA QRLAEEKAQA SSIPVGSRCE VRAAGQSPRR GTVMYVGLTD FKPGYWIGVR YDEPLGKNDG SVNGKRYFEC QAKYGAFVKP AVVTVGDFPE EDYGLDEI |
