STX6 Human

Data sheet

Formulation

The STX6 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 0.2M NaCl, 2mM DTT and 50% glycerol.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Description

STX6 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 257 amino acids (1-234) and having a molecular mass of 29.2kDa.STX6 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatogr

Protein Background

STX6 is a member of the syntaxin family and has 1 t-SNARE coiled-coil homology domain. STX6 takes part in intracellular vesicle transferring. STX6 binds EEA1 and Interacts with VPS45A, MARCH2, MARCH3 and GOPC. It is found in a complex comprising STX6, STX

Expression host

Escherichia Coli.

Synonyms

Syntaxin 6.

Reagent Appearance

Sterile Filtered clear solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSMSMEDPF FVVKGEVQKA VNTAQGLFQR WTELLQDPST ATREEIDWTT NELRNNLRSI EWDLEDLDET ISIVEANPRK FNLDATELSI RKAFITSTRQ VVRDMKDQMS TSSVQALAER KNRQALLGDS GSQNWSTGTT DKYGRLDREL QRANSHFIEE QQAQQQLIVE QQDEQLELVS GSIGVLKNMS QRIGGELEEQ AVMLEDFSHE LE

Product Description

Recombinant SAG2 is effective in detecting IgG to Toxoplasma gondii in human patients with acute toxoplasmosis. It can be used as an antigen in ELISA to test T. gondii infection in domestic cats as well.

Dilution

Toxoplasma protein was purified by proprietary chromatographic technique.