SPRY4 Human

Data sheet

Formulation

The SPRY4 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 0.4M Urea.

Purity

Greater than 85.0% as determined by SDS-PAGE.

Description

SPRY4 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 322 amino acids (1-299) and having a molecular mass of 34.9 kDa.SPRY4 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromat

Protein Background

Sprouty Homolog 4 (SPRY4) is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. SPRY4 is positioned upstream of RAS activation. SPRY4 protein functions include suppressing the insulin receptor and EGFR-trans

Expression host

Escherichia Coli.

Synonyms

Protein sprouty homolog 4, SPRY4, Sprouty Homolog 4, Spry-4, Protein sprouty homolog 4 isoform 2, Sprouty homolog 4 (Drosophila).

Reagent Appearance

Sterile Filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSMEPPIPQ SAPLTPNSVM VQPLLDSRMS HSRLQHPLTI LPIDQVKTSH VENDYIDNPS LALTTGPKRT RGGAPELAPT PARCDQDVTH HWISFSGRPS SVSSSSSTSS DQRLLDHMAP PPVADQASPR AVRIQPKVVH CQPLDLKGPA VPPELDKHFL LCEACGKCKC KECASPRTLP SCWVCNQECL CSAQTLVNYG TCMCLVQGIF YH