SERPINB2 Human

Data sheet

Formulation

Lyophilized from a 0.2um filtered concentrated solution in 20mM Tris-HCl, pH8.0, 150mM NaCl, 1mM Cysteine, with 5% Trehalose.

Solubility

It is recommended to reconstitute the lyophilized SERPINB2 in sterile 18M?-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

SERPINB2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 415 amino acids and having a molecular mass of 46.6kDa.The SERPINB2 is purified by proprietary chromatographic techniques.

Protein Background

SERPINB2 is an inhibitory serpin produced primarily in keratinocytes, stimulated monocytes, and placental trophoblasts. SERPINB2 is found primarily as a 47 kDa non-glycosylated intracellular protein that is induced to be secreted as 60 kDa glycoprotein. T

Expression host

Escherichia Coli.

Synonyms

Serpin Peptidase Inhibitor Clade B (Ovalbumin) Member 2, Serine (Or Cysteine) Proteinase Inhibitor Clade B (Ovalbumin) Member 2, Placental Plasminogen Activator Inhibitor, Plasminogen Activator Inhibitor Type II (Arginine-Serpin), Urokinase Inhibitor, PAI

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized SERPINB2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SERPINB2 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to

Amino acid sequence

MEDLCVANTL FALNLFKHLA KASPTQNLFL SPWSISSTMA MVYMGSRGST EDQMAKVLQF NEVGANAVTP MTPENFTSCG FMQQIQKGSY PDAILQAQAA DKIHSSFRSL SSAINASTGN YLLESVNKLF GEKSASFREE YIRLCQKYYS SEPQAVDFLE CAEEARKKIN SWVKTQTKGK IPNLLPEGSV DGDTRMVLVN AVYFKGKWKT PFEKKLNGLY PFRVNSAQRT PV

Biological Activity

The biologically active was determined by its inhibitory effect against single chain tPA induced cleavage of a chromogenic substrate in Imidazole Buffer at 37°C. Half maximal inhibition against 1.0 ug/ml of single chain tPA was at a concentration of 1.0ug