| Formulation |
The SEC22B solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 2M Urea, 0.15M NaCl and 10% glycerol. |
| Purity |
Greater than 90% as determined by SDS-PAGE. |
| Description |
SEC22B Human Recombinant produced in E. coli is a single polypeptide chain containing 205 amino acids (14-194) and having a molecular mass of 23.3 kDa.SEC22B is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
Vesicle-trafficking protein SEC22b precursor (SEC22B) is a member of the synaptobrevin family. SEC22B forms a complex with SNARE and it is believed to have a role in the ER-Golgi protein trafficking. SEC22B has a strong similarity to the mouse and Chinese hamster proteins. |
| Expression host |
E.coli. |
| Synonyms |
SEC22 Vesicle Trafficking Protein Homolog B (S. Cerevisiae) Gene/Pseudogene, SEC22 Vesicle Trafficking Protein-Like 1 (S. Cerevisiae), ER-Golgi SNARE Of 24 KDa, SEC22L1, ERS-24. |
| Reagent Appearance |
Sterile Filtered colorless solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSM LPLAAS MQEDEQSGRD LQQYQSQAKQ LFRKLNEQSP TRCTLEAGAM TFHYIIEQGV CYLVLCEAAF PKKLAFAYLE DLHSEFDEQH GKKVPTVSRP YSFIEFDTFI QKTKKLYIDS RARRNLGSIN TELQDVQRIM VANIEEVLQR GEALSALDSK ANNLSSLSKK YRQDAKYLNM RSTYA |
| Enzymatic activity |
-3 U/mg protein (Azocoll, 25 °C, 24 h, pH 8.0). |
