| Formulation |
SDHAF1 protein solution (0.25mg/ml) containing Phosphate buffered saline (pH7.4), 30% glycerol, 2mM DTT and 0.1mM PMSF. |
| Purity |
Greater than 85.0% as determined by SDS-PAGE. |
| Description |
SDHAF1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 138 amino acids (1-115 a.a) and having a molecular mass of 15.2kDa. SDHAF1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
Succinate dehydrogenase assembly factor 1 (SDHAF1) is a member of the complex I LYR family. SDHAF1 plays an important role in succinate dehydrogenase complex (SDH) assembly, a complex which is involved in complex II of the mitochondrial electron transport chain. SDHAF1 functions through taking part in mitochondrial biosynthesis of iron-sulfur centers for complex II. |
| Expression host |
Escherichia Coli. |
| Synonyms |
Succinate Dehydrogenase Complex Assembly Factor 1, LYR Motif-Containing Protein 8, LYR Motif Containing 8, SDH Assembly Factor 1, LYRM8, Succinate Dehydrogenase Assembly Factor 1, Mitochondrial, Succinate dehydrogenase assembly factor 1, mitochondrial. |
| Reagent Appearance |
Sterile Filtered colorless solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSMSRHSRL QRQVLSLYRD LLRAGRGKPG AEARVRAEFR QHAGLPRSDV LRIEYLYRRG RRQLQLLRSG HATAMGAFVR PRAPTGEPGG VGSQPDDGDS PRNPHDSTGA PETRPDGR. |
