| Storage |
SARS Mosaic protein is shipped at ambient temperature. Upon arrival, store at -20C. |
| Formulation |
25mM Tris-HCl, 0.4% sarcosyl, 0.25% Triton 100 and 50% glycerol. |
| Purity |
SARS Mosaic protein is >95% pure as determined by 10% PAGE (Coomassie staining). |
| Applications |
SARS Mosaic Antigen in ELISA and Western blots, excellent antigen for detection of SARS with minimal specificity problems. |
| Description |
The E.Coli derived 38 kDa recombinant mosaic protein contains the middle section of the Spike protein 408-470, 540-573 amino acids immunodominant regions. |
| Protein Background |
SARS Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane (M), envelope (E), and spike (S) proteins. Spike (S)-glycoprotein of the virus interacts with a cellular receptor and mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle and is the primary target of neutralizing antibodies. |
| Purification Method |
SARS Mosaic protein was purified by proprietary chromatographic technique. |
