| Storage |
SARS Core protein is shipped at ambient temperature. Upon arrival, store at -20C. |
| Formulation |
50mM Tris-HCl, 60mM NaCl and 50% glycerol. |
| Purity |
SARS Core protein is >95% pure as determined by 10% PAGE (coomassie staining). |
| Applications |
SARS Core antigen is suitable for ELISA and Western blots, excellent antigen for detection of SARS with minimal specificity problems. |
| Description |
The E.Coli derived 35 kDa recombinant protein contains Nucleocapsid protein 1-49, 192-220 amino acids immunodominant regions. The 2 regions are separated with 3 glycine residues. |
| Protein Background |
SARS Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane (M), envelope (E), and spike (S) proteins. Spike (S)-glycoprotein of the virus interacts with a cellular receptor and mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle and is the primary target of neutralizing antibodies. |
| Purification Method |
SARS Core protein was purified by proprietary chromatographic technique. |
