| Formulation |
S100A5 protein (1mg/ml) is supplied in 20mM Tris-HCl buffer, pH8.0, 40% glycerol, 3mM DTT and 200mM NaCl. |
| Purity |
Greater than 95.0% as determined by SDS-PAGE. |
| Description |
S100A5 Mouse Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 117 amino acids (1-93) and having a molecular mass of 13.4kDa.The S100A5 is fused to a 24 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques. |
| Protein Background |
S100 calcium binding protein A5 (S100A5) belongs to the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 family members are localized in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A5 has a Ca2+ affinity 20-100 fold higher than the other S100 proteins investigated under identical conditions. Furthermore, S100A5 protein binds Zn2+ and Cu2+, and Cu2+ strongly which harms the binding of Ca2+. S100A5 is expressed in very limited regions of the adult brain. |
| Expression host |
Escherichia Coli. |
| Synonyms |
Protein S100-A5, Protein S-100D, S100 calcium-binding protein A5, S100a5, S100d, S100D9. |
| Reagent Appearance |
S100A9 is supplied as a sterile filtered clear solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSHMETPLE KALTTMVTTF HKYSGREGSK LTLSRKELKE LIKTELSLAE KMKESSIDNL MKSLDKNSDQ EIDFKEYSVF LTTLCMAYND FFLEDNK. |
