| Formulation |
RSPO1 protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol. |
| Purity |
Greater than 90.0% as determined by SDS-PAGE. |
| Description |
RSPO1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 254 amino acids (21-265a.a.) and having a molecular mass of 28.2kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa). RSPO1 is expressed with an 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques. |
| Protein Background |
R-Spondin-1 (Rspo1) is a part of the Rspondin family. Rspo1 plays a role as an activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Rspo1 induces the onset of crypt cell proliferation and increases intestinal epithelial healing effect. Rspo1 is negatively regulating the TGF-beta pathway. |
| Expression host |
Sf9, Baculovirus cells. |
| Synonyms |
R-spondin-1, Cristin-3, mCristin-3, Roof plate-specific spondin-1, Rspo1, RSPO, R-spondin 1, R-spondin, Rspondin, CRISTIN3. |
| Reagent Appearance |
Sterile Filtered colorless solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
ADPSRGIKGK RQRRISAEGS QACAKGCELC SEVNGCLKCS PKLFILLERN DIRQVGVCLP SCPPGYFDAR NPDMNKCIKC KIEHCEACFS HNFCTKCQEG LYLHKGRCYP ACPEGSTAAN STMECGSPAQ CEMSEWSPWG PCSKKRKLCG FRKGSEERTR RVLHAPGGDH TTCSDTKETR KCTVRRTPCP EGQKRRKGGQ GRRENANRHP ARKNSKEPGS NSRRHKGQQQ PQPGTTGPLT SVGPTWAQHH HHHH. |
| Quality Control Test |
MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVTTVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA. |
