RPS3A Human

Data sheet

Formulation

The RPS3A solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 200mM NaCl, 2mM DTT and 50% glycerol.

Purity

Greater than 90% as determined by SDS-PAGE.

Description

RPS3A Human Recombinant produced in E. coli is a single polypeptide chain containing 288 amino acids (1-264) and having a molecular mass of 32.5kDa.RPS3A is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic technique

Protein Background

RPS3A is a member of the S3AE family of ribosomal proteins. Ribosomes, the organelles which catalyze protein synthesis, composed of a large 60S subunit and a small 40S subunit. Together these subunits consist of 4 RNA classes and around 80 structurally di

Expression host

E.coli.

Synonyms

Ribosomal protein S3A, v-fos transformation effector protein 1, 40S ribosomal protein S3a, MFTL, FTE1, S3A.

Reagent Appearance

Sterile Filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSHMAVGKN KRLTKGGKKG AKKKVVDPFS KKDWYDVKAP AMFNIRNIGK TLVTRTQGTK IASDGLKGRV FEVSLADLQN DEVAFRKFKL ITEDVQGKNC LTNFHGMDLT RDKMCSMVKK WQTMIEAHVD VKTTDGYLLR LFCVGFTKKR NNQIRKTSYA QHQQVRQIRK KMMEIMTREV QTNDLKEVVN KLIPDSIGKD IEKACQSIYP LH

Quality Control Test

MSGDDDIPEG LEAINLKMNA TTDDSLESTR RMLALCEESK EAGIKTLVML DDQGEQLERCEGALDTINQD MKEAEDHLKG MEKCCGLCVL PWNKTDDFEK TEFAKAWKKD DDGGVISDQPRITVGDSSMG PQGGYITKIT NDAREDEMDE NVQQVSTMVG NLRNMAIDMS TEVSNQNRQL DRIHDKAQSN EVRVESANKR AKNLITK.