| Formulation |
RNASE7 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0) and 10% glycerol 1mM DTT. |
| Purity |
Greater than 90% as determined by SDS-PAGE. |
| Description |
RNASE7 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 151 amino acids (29-156 a.a) and having a molecular mass of 16.9kDa. RNASE7 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
Ribonuclease 7 (RNASE7) is one of the final RNase A superfamily ribonucleases. RNASE7 was isolated from skin-derived stratum corneum. RNASE7 protein demonstrated potent ribonuclease activity and hence may contribute to the well-known ribonuclease activity of human skin. RNASE7 has revealed a broad spectrum antimicrobial activity against many pathogenic microorganisms and extraordinarily potent activity against a vancomycin-resistant Enterococcus faecium. |
| Expression host |
Escherichia Coli. |
| Synonyms |
Ribonuclease, RNase A Family, 7, Skin-Derived Antimicrobial Protein 2, RNase 7, SAP-2, EC 3.1.27.5, EC 3.1.27., EC 3.1.27, Ribonuclease 7. |
| Reagent Appearance |
Sterile Filtered colorless solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSKPKGMTS SQWFKIQHMQ PSPQACNSAM KNINKHTKRC KDLNTFLHEP FSSVAATCQT PKIACKNGDK NCHQSHGPVS LTMCKLTSGK YPNCRYKEKR QNKSYVVACK PPQKKDSQQF HLVPVHLDRV L. |
