| Formulation |
RASD2 protein solution (0.5mg/ml) containing 20mM Tris-HCl (pH8.0) and 10% glycerol. |
| Purity |
Greater than 85.0% as determined by SDS-PAGE. |
| Inactivation |
Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.06 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of SDF-1b as a Reference Standard. |
| Description |
RASD2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 289 amino acids (1-266 a.a) and having a molecular mass of 32.8 kDa.RASD2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
RASD Family Member 2 (RASD2) which binds to GTP and owns a GTPase activity is a part of the Ras super family of small GTPases. RASD2 regulates signaling pathways involving G-proteins-coupled receptor and heterotrimeric proteins. RASD2 encodes a Ras-related protein that enriched in striatum. |
| Expression host |
Escherichia Coli. |
| Synonyms |
GTP-binding protein Rhes, Ras homolog enriched in striatum, TEM2, RASD2, Rhes, Tumor endothelial marker 2, RASD Family Member 2. |
| Reagent Appearance |
Sterile Filtered clear solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSMMKTLSS GNCTLSVPAK NSYRMVVLGA SRVGKSSIVS RFLNGRFEDQ YTPTIEDFHR KVYNIRGDMY QLDILDTSGN HPFPAMRRLS ILTGDVFILV FSLDNRESFD EVKRLQKQIL EVKSCLKNKT KEAAELPMVI CGNKNDHGEL CRQVPTTEAE LLVSGDENCA YFEVSAKKNT NVDEMFYVLF SMAKLPHEMS PALHRKISVQ YGDAFHPRPF CMRRVKEMDA YGMVSPFARR PSVNSDLKYI KAKVLREGQA RERDKCTIQ. |
