RANBP1 Human

Data sheet

Formulation

The RANBP1 solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl, 1mM DTT and 10% glycerol.

Purity

Greater than 95% as determined by SDS-PAGE.

Inactivation

Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.52 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program o

Description

RANBP1 Human Recombinant produced in E. coli is a single polypeptide chain containing 225 amino acids (1-201) and having a molecular mass of 25.8kDa (molecular size on SDS-PAGE will appear higher).RANBP1 is fused to a 24 amino acid His-tag at N-terminus &

Protein Background

RANBP1 is a member of a family of proteins which bind Ran GTPase. Ran GTPase is vital for cell cycle control, mRNA processing, nuclear transport, mitotic spindle assembly, and postmitotic nuclear re-assembly and nuclear architecture maintenance. RANBP1 do

Expression host

E.coli.

Synonyms

RAN binding protein 1, HTF9A, ran-specific GTPase-activating protein, RanBP1.

Reagent Appearance

Sterile Filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSHMAAAKD THEDHDTSTE NTDESNHDPQ FEPIVSLPEQ EIKTLEEDEE ELFKMRAKLF RFASENDLPE WKERGTGDVK LLKHKEKGAI RLLMRRDKTL KICANHYITP MMELKPNAGS DRAWVWNTHA DFADECPKPE LLAIRFLNAE NAQKFKTKFE ECRKEIEERE KKAGSGKNDH AEKVAEKLEA LSVKEETKED AEEKQ.