| Formulation |
The PTGR1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer, pH7.5, 10% glycerol, 1mM DTT and 200mM NaCl. |
| Purity |
Greater than 90.0% as determined by SDS-PAGE. |
| Description |
PTGR1 Human Recombinant produced in E. coli is a single polypeptide chain containing 354 amino acids (1-329) and having a molecular mass of 38.6kDa.PTGR1 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
Prostaglandin Reductase 1 (PTGR1) is a member of the NADP-dependent oxidoreductase L4BD family. PTGR1 catalyzes the conversion of leukotriene B4 into its biologically less active metabolite, 12-oxo-leukotriene B4, thus being a primary step of metabolic inactivation of leukotriene B4. PTGR1 is highly expressed in the kidney, liver, and intestine but not in leukocytes. |
| Expression host |
Escherichia Coli. |
| Synonyms |
Prostaglandin reductase 1, PRG-1, 15-oxoprostaglandin 13-reductase, NADP-dependent leukotriene B4 12-hydroxydehydrogenase, PTGR1, LTB4DH, PGR1, ZADH3. |
| Reagent Appearance |
Sterile filtered colorless solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSEFMVRTK TWTLKKHFVG YPTNSDFELK TAELPPLKNG EVLLEALFLT VDPYMRVAAK RLKEGDTMMG QQVAKVVESK NVALPKGTIV LASPGWTTHS ISDGKDLEKL LTEWPDTIPL SLALGTVGMP GLTAYFGLLE ICGVKGGETV MVNAAAGAVG SVVGQIAKLK GCKVVGAVGS DEKVAYLQKL GFDVVFNYKT VESLEETLKK ASPDGYDCYF DNVGGEFSNT VIGQMKKFGR IAICGAISTY NRTGPLPPGP PPEIVIYQEL RMEAFVVYRW QGDARQKALK DLLKWVLEGK IQYKEYIIEG FENMPAAFMG MLKGDNLGKT IVKA. |
