PRKAB1 Human

Data sheet

Formulation

The PRKAB1 protein solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.

Purity

Greater than 85% as determined by SDS-PAGE.

Description

PRKAB1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 293 amino acids (1-270 a.a.) and having a molecular mass of 32.8 kDa. The PRKAB1 is fused to a 23 amino acid His Tag at N-Terminus and purified by prop

Protein Background

5'-AMP-activated protein kinase subunit beta-1 (PRKAB1) hinders protein, carbohydrate and lipid biosynthesis, in addition to cell growth and proliferation. AMPK is a heterotrimer comprised of an alpha catalytic subunit, and non-catalytic beta and gamma su

Expression host

E.coli.

Synonyms

AMPK, HAMPKb, 5'-AMP-activated protein kinase subunit beta-1, AMPK subunit beta-1, AMPKb,PRKAB1.

Reagent Appearance

Sterile Filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSMGNTSSE RAALERHGGH KTPRRDSSGG TKDGDRPKIL MDSPEDADLF HSEEIKAPEK EEFLAWQHDL EVNDKAPAQA RPTVFRWTGG GKEVYLSGSF NNWSKLPLTR SHNNFVAILD LPEGEHQYKF FVDGQWTHDP SEPIVTSQLG TVNNIIQVKK TDFEVFDALM VDSQKCSDVS ELSSSPPGPY HQEPYVCKPE ERFRAPPILP PH