| Formulation |
POP4 protein solution (1mg/ml) contains 20mM Tris-HCl buffer, (pH 8.0), 0.4M UREA and 10% glycerol. |
| Purity |
Greater than 85.0% as determined by SDS-PAGE. |
| Description |
POP4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 243 amino acids (1-220 a.a.) and having a molecular mass of 27.8kDa.POP4 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
Processing of Precursor 4 (POP4) is a member of the eukaryotic/archaeal RNase P protein component 1 family. POP4 is one of the protein subunits of the small nucleolar ribonucleoprotein complexes: the endoribonuclease for mitochondrial RNA processing complex and the ribonuclease P complex. POP4 is confined to the nucleus and associates promptly with the RNA component of this complexes.POP4 is participating in processing of precursor RNAs. |
| Expression host |
Escherichia Coli. |
| Synonyms |
RPP29, Ribonuclease P protein subunit p29, hPOP4. |
| Reagent Appearance |
Sterile Filtered clear solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSMKSVIYH ALSQKEANDS DVQPSGAQRA EAFVRAFLKR STPRMSPQAR EDQLQRKAVV LEYFTRHKRK EKKKKAKGLS ARQRRELRLF DIKPEQQRYS LFLPLHELWK QYIRDLCSGL KPDTQPQMIQ AKLLKADLHG AIISVTKSKC PSYVGITGIL LQETKHIFKI ITKEDRLKVI PKLNCVFTVE TDGFISYIYG SKFQLRSSER SAKKFKAKGT IDL. |
