| Formulation |
POLR3K protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 50% glycerol, 2mM DTT and 2mM EDTA. |
| Purity |
Greater than 85.0% as determined by SDS-PAGE. |
| Description |
POLR3K Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 131 amino acids (1-108 a.a) and having a molecular mass of 14.7kDa.POLR3K is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
Polymerase III Polypeptide K, known as PORL3K, is a small vital subunit of RNA polymerase III. The carboxy-terminal domain of PORL3K owns a sequence which is very similar to the carboxy-terminal domain of an RNA polymerase II elongation factor. PORL3K takes part in sensing and limiting infection by intracellular bacteria and DNA viruses. PORL3K is also plays a role as nuclear and cytosolic DNA sensor which takes part in the innate immune response. |
| Expression host |
Escherichia Coli. |
| Synonyms |
DNA-directed RNA polymerase III subunit RPC10, RNA polymerase III subunit C10, DNA-directed RNA polymerase III subunit K, RNA polymerase III 12.5 kDa subunit, RPC12.5, RNA polymerase III subunit C11, HsC11p, RPC11, hRPC11, POLR3K, My010, Polymerase III Polypeptide K, C11, C11-RNP3, hRPC11, RPC10. |
| Reagent Appearance |
Sterile Filtered colorless solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSMLLFCPG CGNGLIVEEG QRCHRFACNT CPYVHNITRK VTNRKYPKLK EVDDVLGGAA AWENVDSTAE SCPKCEHPRA YFMQLQTRSA DEPMTTFYKC CNAQCGHRWR D. |
