POLR3H Human

Data sheet

Formulation

POLR3H protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 10% glycerol and 1mM DTT.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Description

POLR3H Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 227 amino acids (1-204 a.a.) and having a molecular mass of 25.3kDa (Molecular size on SDS-PAGE will appear higher).POLR3H is fused to a 23 amino acid H

Protein Background

Polymerase (RNA) III (DNA directed) Polypeptide H(POLR3H) is a member of the eukaryotic RPB7/RPC8 RNA polymerase subunit family. POLR3H is DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA utilizing the 4 ribonucleoside tripho

Expression host

Escherichia Coli.

Synonyms

DNA-directed RNA polymerase III subunit RPC8, RNA polymerase III subunit C8, DNA-directed RNA polymerase III subunit H, RNA polymerase III subunit 22.9 kDa subunit, RPC22.9, POLR3H, KIAA1665, RPC8.

Reagent Appearance

Sterile Filtered clear solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSMFVLVEM VDTVRIPPWQ FERKLNDSIA EELNKKLANK VVYNVGLCIC LFDITKLEDA YVFPGDGASHTKVHFRCVVF HPFLDEILIG KIKGCSPEGV HVSLGFFDDI LIPPESLQQP AKFDEAEQVW VWEYETEEGA HDLYMDTGEE IRFRVVDESF VDTSPTGPSS ADATTSSEEL PKKEAPYTLV GSISEPGLGL LSWWTSN.