POLR2F Human

Data sheet

Formulation

The POLR2F solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT.

Purity

Greater than 95% as determined by SDS-PAGE.

Description

POLR2F Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 150 amino acids (1-127) and having a molecular mass of 16.9kDa (Molecular size on SDS-PAGE will appear higher).POLR2F is fused to a 23 amino acid His-ta

Protein Background

Polymerase II Polypeptide F (POLR2F) is the 6th largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes, which is also shared by the other two DNA-directed RNA polymerases. In yeast, POLR2F, in combina

Expression host

E.coli.

Synonyms

Polymerase (RNA) II (DNA Directed) Polypeptide F, DNA Directed RNA Polymerase II 14.4 Kda Polypeptide, POLRF, RPABC14.4, RPB14.4, RPC15, DNA-Directed RNA Polymerase II Subunit F, DNA-Directed RNA Polymerases I, II, And III 14.4 KDa Polypeptide, RNA Polyme

Reagent Appearance

Sterile Filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSMSDNEDN FDGDDFDDVE EDEGLDDLEN AEEEGQENVE ILPSGERPQA NQKRITTPYM TKYERARVLG TRALQIAMCA PVMVELEGET DPLLIAMKEL KARKIPIIIR RYLPDGSYED WGVDELIITD.