| Formulation |
PLGF-2 protein was lyophilized from a 0.2um filtered solution in HCl. |
| Solubility |
It is recommended to reconstitute the lyophilized PLGF2 at 200ug/ml in PBS, which can then be further diluted to other aqueous solutions. |
| Purity |
Greater than 95.0% as determined by SDS-PAGE. |
| Description |
PLGF2 Human Recombinant (19-170 a.a.) produced in CHO is a disulfide-linked homodimeric, glycosylated, polypeptide chain containing 152 amino acids and having a molecular mass of 33kDa.The PLGF-2 Human Recombinant protein is purified by proprietary chromatographic techniques. |
| Protein Background |
PLGF is a growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration. It binds to receptor vegfr-1/flt1.PLGF-2 binds neuropilin-1 and 2 in a heparin-dependent manner. |
| Expression host |
Chinese Hamster Ovary cells (CHO). |
| Synonyms |
PIGF, PGF, PlGF-2, PLGF-2. |
| Reagent Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability |
Lyophilized PLGF-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PLGF2 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Amino acid sequence |
LPAVPPQQWA LSAGNGSSEV EVVPFQEVWG RSYCRALERL VDVVSEYPSE VEHMFSPSCV SLLRCTGCCG DENLHCVPVE TANVTMQLLK IRSGDRPSYV ELTFSQHVRC ECRPLREKMK PERRRPKGRG KRRREKQRPT DCHLCGDAVP RR. |
| Biological Activity |
The ED50, as measured in a cell proliferation assay using MDA?MB?231 cells is less than 10ug/ml. |
