PLGF2 Human, CHO

Data sheet

Formulation

PLGF-2 protein was lyophilized from a 0.2um filtered solution in HCl.

Solubility

It is recommended to reconstitute the lyophilized PLGF2 at 200ug/ml in PBS, which can then be further diluted to other aqueous solutions.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Description

PLGF2 Human Recombinant (19-170 a.a.) produced in CHO is a disulfide-linked homodimeric, glycosylated, polypeptide chain containing 152 amino acids and having a molecular mass of 33kDa.The PLGF-2 Human Recombinant protein is purified by proprietary chroma

Protein Background

PLGF is a growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration. It binds to receptor vegfr-1/flt1.PLGF-2 binds neuropilin-1 and 2 in a heparin-dependent manner.

Expression host

Chinese Hamster Ovary cells (CHO).

Synonyms

PIGF, PGF, PlGF-2, PLGF-2.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized PLGF-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PLGF2 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add

Amino acid sequence

LPAVPPQQWA LSAGNGSSEV EVVPFQEVWG RSYCRALERL VDVVSEYPSE VEHMFSPSCV SLLRCTGCCG DENLHCVPVE TANVTMQLLK IRSGDRPSYV ELTFSQHVRC ECRPLREKMK PERRRPKGRG KRRREKQRPT DCHLCGDAVP RR.

Biological Activity

The ED50, as measured in a cell proliferation assay using MDA?MB?231 cells is less than 10ug/ml.