PLGF1 Human

Data sheet

Formulation

PLGF-1 protein was lyophilized from a 0.2um filtered solution in Acetonitrile and TFA.

Solubility

It is recommended to reconstitute the lyophilized PLGF1 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 97.0% as determined by SDS-PAGE.

Description

PLGF1 Human Recombinant (21-221 a.a.) produced in E.Coli is a disulfide-linked homodimeric, non-glycosylated, polypeptide chain containing 129 amino acids and having a molecular mass of 29kDa.The PLGF-1 Human Recombinant protein is purified by proprietary

Protein Background

PLGF is a growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration. It binds to receptor vegfr-1/flt1.

Expression host

Escherichia coli.

Synonyms

PIGF, PGF, PLGF-1, PlGF-2.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized PLGF-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PLGF1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add

Amino acid sequence

AVPPQQWALS AGNGSSEVEV VPFQEVWGRS YCRALERLVD VVSEYPSEVE HMFSPSCVSL LRCTGCCGDE NLHCVPVETA NVTMQLLKIR SGDRPSYVEL TFSQHVRCEC RPLREKMKPE RCGDAVPRR.

Biological Activity

Binds to immobilized rHuVEGFR1 with EC50 less than 6ng/ml.