PINK1 Human

Data sheet

Formulation

The PINK1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1M Urea and 5% glycerol.

Purity

Greater than 90% as determined by SDS-PAGE.

Description

PINK1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 353 amino acids (156-507) and having a molecular mass of 37.9 kDa.PINK1 is purified by proprietary chromatographic techniques.

Protein Background

PINK1 is a serine/threonine protein kinase which is found in the mitochondria. PINK1 shields cells from stress-induced mitochondrial dysfunction. Mutations results in one form of autosomal recessive early-onset Parkinson disease.

Expression host

E.coli.

Synonyms

PTEN induced putative kinase 1, PARK6, protein kinase BRPK, Parkinson disease (autosomal recessive) 6, serine/threonine-protein kinase PINK1 mitochondrial, EC 2.7.11.1.

Reagent Appearance

Sterile Filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MYLIGQSIGK GCSAAVYEAT MPTLPQNLEV TKSTGLLPGR GPGTSAPGEG QERAPGAPAF PLAIKMMWNI SAGSSSEAIL NTMSQELVPA SRVALAGEYG AVTYRKSKRG PKQLAPHPNI IRVLRAFTSS VPLLPGALVD YPDVLPSRLH PEGLGHGRTL FLVMKNYPCT LRQYLCVNTP SPRLAAMMLL QLLEGVDHLV QQGIAHRDLK SDNILVELDP DGCPWLVIAD FG