PhI p 2

Data sheet

Formulation

PhI p 2 is supplied in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol.

Purity

Greater than 80.0% as determined by SDS-PAGE.

Description

Recombinant Pollen allergen Phl p 2 produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 14,734 Dalton. PhI p 2 is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic techniques.

Protein Background

Pollen allergen Phl p 2 (PhI p 2) causes an allergic reaction in humans.

Expression host

Sf9 insect cells.

Synonyms

Pollen allergen Phl p 2, Allergen Phl p II, Phl p 2, PHLPII.

Reagent Appearance

Sterile Filtered clear solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

Immunological functions

1. Binds IgE type human antibodies. 2. Immunodot test with positive/negative sera panels.

Molar extinction coefficient

15470; A280(1mg/ml)=1.269