| Formulation |
The PHF13 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 50% glycerol, 2mM DTT, 1mM EDTA and 250mM Imidazole. |
| Purity |
Greater than 85% as determined by SDS-PAGE. |
| Description |
PHF13 Human Recombinant produced in E. coli is a single polypeptide chain containing 323 amino acids (1-300) and having a molecular mass of 36kDa. PHF13 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
PHD finger protein 13 (PHF13) is necessary for normal chromosome separation during mitosis and also for normal chromosome condensation during the early stages of mitosis. PHF13 modulates chromatin structure. |
| Expression host |
Escherichia Coli. |
| Synonyms |
PHF5, SPOC1, PHD finger protein 13, Survival time-associated PHD finger protein in ovarian cancer 1, PHF13. |
| Reagent Appearance |
Sterile Filtered clear solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSMDSDSCA AAFHPEEYSP SCKRRRTVED FNKFCTFVLA YAGYIPYPKE ELPLRSSPSP ANSTAGTIDS DGWDAGFSDI ASSVPLPVSD RCFSHLQPTL LQRAKPSNFL LDRKKTDKLK KKKKRKRRDS DAPGKEGYRG GLLKLEAADP YVETPTSPTL QDIPQAPSDP CSGWDSDTPS SGSCATVSPD QVKEIKTEGK RTIVRQGKQV VFRDEDSTGN DEDIMVDSDD DSWDLVTCFC MKPFAGRPMI ECNECHTWIH LSCAKIRKSN VPEVFVCQKC RDSKFDIRRS NRSRTGSRKL FLD. |
