| Formulation |
The PET117 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 50% glycerol and 2mM DTT. |
| Purity |
Greater than 90.0% as determined by SDS-PAGE. |
| Description |
PET117 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 82 amino acids (23-81) and having a molecular mass of 9.5kDa.PET117 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
PET117 is nuclear gene essential for the assembly of active cytochrome c oxidase in S. cerevisiae. Nevertheless the gene products are not subunits of the final assembled cytochrome c oxidase complex. PET117 is found in chromosome V proximate the HIS1 gene. |
| Expression host |
Escherichia Coli. |
| Synonyms |
Protein PET117 homolog, mitochondrial, PET117, UNQ607/PRO1194. |
| Reagent Appearance |
Sterile Filtered colorless solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSVHVKQQW DQQRLRDGVI RDIERQIRKK ENIRLLGEQI ILTEQLEAER EKMLLAKGSQ KS. |
