PDHX Human

Data sheet

Formulation

PDHX protein solution (0.25mg/ml) containing Phosphate buffer saline (pH 7.4) and 20% glycerol, 1mM DTT.

Purity

Greater than 85.0% as determined by SDS-PAGE.

Description

PDHX Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 471 amino acids (54-501 a.a) and having a molecular mass of 50.4kDa. PDHX is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chro

Protein Background

Pyruvate Dehydrogenase Complex, Component X, also known as PDHX, encodes the E3 binding protein subunit of the PDH complex which contains 3 catalytic subunits. PDHX tethers E3 dimers to the E2 core of the pyruvate dehydrogenase complexes of eukaryotes. Th

Expression host

Escherichia Coli.

Synonyms

DLDBP, E3BP, OPDX, PDX1, proX, Pyruvate Dehydrogenase Complex, Component X, Dihydrolipoamide Dehydrogenase-Binding Protein Of Pyruvate Dehydrogenase Complex, Lipoyl-Containing Pyruvate Dehydrogenase Complex Component X, Pyruvate Dehydrogenase Complex, Lip

Reagent Appearance

Sterile Filtered clear solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSGDPIKIL MPSLSPTMEE GNIVKWLKKE GEAVSAGDAL CEIETDKAVV TLDASDDGIL AKIVVEEGSK NIRLGSLIGL IVEEGEDWKH VEIPKDVGPP PPVSKPSEPR PSPEPQISIP VKKEHIPGTL RFRLSPAARN ILEKHSLDAS QGTATGPRGI FTKEDALKLV QLKQTGKITE SRPTPAPTAT PTAPSPLQAT AGPSYPRPVI PP