PARD6B Human

Data sheet

Formulation

The PARD6B solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 2M Urea, 20% glycerol and 0.2M NaCl.

Purity

Greater than 85.0% as determined by SDS-PAGE.

Description

PARD6B Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 395 amino acids (1-372) and having a molecular mass of 43.6kDa.PARD6B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chroma

Protein Background

Partitioning defective 6 homolog beta (PARD6B) belongs to the PAR6 family and encodes a protein with a PSD95/Discs-large/ZO1 (PDZ) domain, an OPR domain and a semi-Cdc42/Rac interactive binding (CRIB) domain. Cellular asymmetry is crucial for the developm

Expression host

Escherichia Coli.

Synonyms

Partitioning defective 6 homolog beta, PAR-6 beta, PAR-6B, PARD6B, PAR6B.

Reagent Appearance

Sterile Filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSMNRSHRH GAGSGCLGTM EVKSKFGAEF RRFSLERSKP GKFEEFYGLL QHVHKIPNVD VLVGYADIHG DLLPINNDDN YHKAVSTANP LLRIFIQKKE EADYSAFGTD TLIKKKNVLT NVLRPDNHRK KPHIVISMPQ DFRPVSSIID VDILPETHRR VRLYKYGTEK PLGFYIRDGS SVRVTPHGLE KVPGIFISRL VPGGLAQSTG LL