P4HB Human, Active

Data sheet

Formulation

The P4HB 1mg/ml protein solution contains 20mM Tris-HCl pH-8, and 10% glycerol.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Description

P4HB Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 512 amino acids (18-508 a.a.) and having a molecular mass of 57.5kDa. The P4HB is fused to a 21 amino acid His Tag and purified by conventional chromatogr

Protein Background

P4HB is a multifunctional and highly abundant enzyme that is part of the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, P4HB has a role in hydroxylation of prolyl residues in preprocollagen. P

Expression host

Escherichia Coli.

Synonyms

P4Hbeta, PDI, PDIA1, PHD, PO4DB, PO4HB, ERBA2L.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MDAPEEEDHV LVLRKSNFAE ALAAHKYLLV EFYAPWCGHC KALAPEYAKA AGKLKAEGSE IRLAKVDATE ESDLAQQYGV RGYPTIKFFR NGDTASPKEY TAGREADDIV NWLKKRTGPA ATTLPDGAAA ESLVESSEVA VIGFFKDVES DSAKQFLQAA EAIDDIPFGI TSNSDVFSKY QLDKDGVVLF KKFDEGRNNF EGEVTKENLL DF

Physical Appearence

Sterile Filtered colorless solution.

Biological Activity

Specific activity > 100 A650/cm/min/mg. Enzymatic activity was confirmed by measuring the aggregation of insulin in the presence of DTT.