OVCA2 Human

Data sheet

Reconstitution

1mg/ml in PBS (after reconstitution).

Formulation

The OVCA2 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM Nacl, 1mM DTT and 10% glycerol.

Purity

Greater than 90% as determined by SDS-PAGE.

Description

OVCA2 Human Recombinant produced in E. coli is a single polypeptide chain containing 251 amino acids (1-227) and having a molecular mass of 26.9kDa.OVCA2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic technique

Protein Background

OVCA2 is a member of the UPF0483 family. Universally expressed, the OVCA2 expression is regulated by retinoids. OVCA2 cooperates with apoptosis-related protein Bag-1, which indicates that OVCA2 takes part in cell proliferation. OVCA2 is thought to perform

Expression host

E.coli.

Synonyms

Ovarian tumor suppressor candidate 2, candidate tumor suppressor in ovarian cancer 2, ovarian cancer-associated gene 2 protein.

Reagent Appearance

Sterile Filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSHMAAQRP LRVLCLAGFR QSERGFREKT GALRKALRGR AELVCLSGPH PVPDPPGPEG ARSDFGSCPP EEQPRGWWFS EQEADVFSAL EEPAVCRGLE ESLGMVAQAL NRLGPFDGLL GFSQGAALAA LVCALGQAGD PRFPLPRFIL LVSGFCPRGI GFKESILQRP LSLPSLHVFG DTDKVIPSQE SVQLASQFPG AITLTHSGGH FI

Antibody Properties

Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Titer

Antibody is shipped lyophilized at ambient temperature.

Protein content

By direct ELISA, 1:10,000 dilution will yield 0.5 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories).