NPHS2 Human

Data sheet

Formulation

NPHS2 was filtered (0.4um) and lyophilized in 30mM acetate buffer and 1mM dithiothreitol, pH 4.

Solubility

It is recommended to add 0.1M acetate buffer pH 4.0 to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer t

Purity

Greater than 95.0% as determined by SDS-PAGE.

Description

NPHS2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (Val125-Leu383) containing 269 amino acids including a 10 aa His tag at N-terminus. The total calculated molecular mass is 30.2kDa.

Protein Background

Nephrosis 2 Idiopathic Steroid-Resistant (NPHS2) is a protein which has a role in the regulation of glomerular permeability, serving as a linker between the plasma membrane and the cytoskeleton. NPHS2 gene mutations cause steroid-resistant nephrotic syndr

Expression host

Escherichia Coli.

Synonyms

Nephrosis 2, Idiopathic, Steroid-Resistant (Podocin), PDCN, SRN1, Podocin, NPHS2.

Reagent Appearance

Filtered White lyophilized (freeze-dried) powder.

Stability

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Amino acid sequence

MKHHHHHHASVKVVQEYERV IIFRLGHLLP GRAKGPGLFF FLPCLDTYHK VDLRLQTLEI PFHEIVTKDM FIMEIDAICY YRMENASLLL SSLAHVSKAV QFLVQTTMKR LLAHRSLTEI LLERKSIAQD AKVALDSVTC IWGIKVERIE IKDVRLPAGL QHSLAVEAEA QRQAKVRMIA AEAEKAASES LRMAAEILSG TPAAVQLRYL HTLQSLSTEK PSTVVLPLPF DLL