| Formulation |
NHLH2 protein solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 7.5), 0.2M NaCl, 50% glycerol and 5mM DTT. |
| Purity |
Greater than 90.0% as determined by SDS-PAGE. |
| Description |
NHLH2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 158 amino acids (1-135 a.a.) and having a molecular mass of 17.4kDa.NHLH2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
Nescient Helix Loop Helix 2 (NHLH2) belongs to the basic helix-loop-helix (bHLH) transcription factor family. NHLH2 is expressed in developing and adult neuroendocrine tissues for example the pituitary and hypothalamus. NHLH2 can act as a DNA-binding protein and may be involved in the control of cell-type determination, probably in the developing nervous system. |
| Expression host |
Escherichia Coli. |
| Synonyms |
nescient helix loop helix 2, bHLHa34, HEN2, NSCL2, Class A basic helix-loop-helix protein 34, NHLH2, KIAA0490. |
| Reagent Appearance |
Sterile Filtered clear solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSMMLSPDQ AADSDHPSSA HSDPESLGGT DTKVLGSVSD LEPVEEAEGD GKGGSRAALY PHPQQLSREE KRRRRRATAK YRSAHATRER IRVEAFNLAF AELRKLLPTL PPDKKLSKIE ILRLAICYIS YLNHVLDV. |
