| Formulation |
The MXD3 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol. |
| Purity |
Greater than 90.0% as determined by SDS-PAGE. |
| Description |
MXD3 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 229 amino acids (1-206) and having a molecular mass of 25.9 kDa. MXD3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
MAX Dimerization Protein 3 (MXD3) belongs to the Myc superfamily of basic helix-loop-helix leucine zipper transcriptional regulators. MXD3 forms a heterodimer with the cofactor MAX that connects specific E-box DNA motifs in the promoters of target genes and regulates their transcription. Disturbance of the MAX-MXD3 complex is correlated with uncontrolled cell proliferation and tumorigenesis. Transcript variants of MXD3 encoding different isoforms have been described. |
| Expression host |
Escherichia Coli. |
| Synonyms |
BHLHC13, MAD3, MYX, Max dimerization protein 3, Max dimerizer 3, Class C basic helix-loop-helix protein 13, bHLHc13, Max-associated protein 3, Max-interacting transcriptional repressor MAD3, MXD3 Human, MXD3. |
| Reagent Appearance |
Sterile Filtered colorless solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSMEPLASN IQVLLQAAEF LERREREAEH GYASLCPHRS PGPIHRRKKR PPQAPGAQDS GRSVHNELEK RRRAQLKRCL ERLKQQMPLG ADCARYTTLS LLRRARMHIQ KLEDQEQRAR QLKERLRSKQ QSLQRQLEQL RGLAGAAERE RLRADSLDSS GLSSERSDSD QEELEVDVES LVFGGEAELL RGFVAGQEHS YSHGGGAWL. |
