| Formulation |
The MT3 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl and 10% glycerol. |
| Purity |
Greater than 85% as determined by SDS-PAGE. |
| Description |
MT3 Human Recombinant produced in E. coli is a single polypeptide chain containing 91 amino acids (1-68) and having a molecular mass of 9.3kDa (molecular size on SDS-PAGE will appear higher).MT3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
Metallothionein 3 (MT3) contains 3 zinc and 3 copper atoms per polypeptide chain and a minor amount of cadmium. MT3 inhibits survival and neurite formation of cortical neurons in vitro. MT3 also binds heavy metals. |
| Expression host |
Escherichia Coli. |
| Synonyms |
GIF, GIFB, GRIF, ZnMT3, Metallothionein-3, MT-3, Growth inhibitory factor, Metallothionein-III, MT-III, MT3. |
| Reagent Appearance |
Sterile Filtered clear solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSMDPETCP CPSGGSCTCA DSCKCEGCKC TSCKKSCCSC CPAECEKCAK DCVCKGGEAA EAEAEKCSCC Q. |
