| Formulation |
MOB1B protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 30% glycerol and 1mM DTT. |
| Purity |
Greater than 95% as determined by SDS-PAGE. |
| Description |
MOB1B Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 239 amino acids (1-216 a.a) and having a molecular mass of 27kDa. MOB1B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
MOB Kinase Activator 1B (MOB1B) is similar to the yeast Mob1 protein. Yeast Mob1 binds Mps1p, which is a protein kinase vital for spindle pole body duplication and mitotic checkpoint regulation. |
| Expression host |
E.coli. |
| Synonyms |
MOB Kinase Activator 1B, Mps One Binder Kinase Activator-Like 1A, Mob1 Homolog 1A, MOBKL1A, Mob1A, MOB4A, MOB1, Mps One Binder Kinase Activator-Like 1A (Yeast), MOB1, Mps One Binder Kinase Activator-Like 1A, MOB1 Mps One Binder Homolog B (Yeast), MOB1 Mps One Binder Homolog B, Mob4A Protein, MATS2, Mob1B, MOB kinase activator 1B. |
| Reagent Appearance |
Sterile Filtered colorless solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSMSFLFGS RSSKTFKPKK NIPEGSHQYE LLKHAEATLG SGNLRMAVML PEGEDLNEWV AVNTVDFFNQ INMLYGTITD FCTEESCPVM SAGPKYEYHW ADGTNIKKPI KCSAPKYIDY LMTWVQDQLD DETLFPSKIG VPFPKNFMSV AKTILKRLFR VYAHIYHQHF DPVIQLQEEA HLNTSFKHFI FFVQEFNLID RRELAPLQEL IEKLTSKDR. |
