| Formulation |
MEP1B protein solution (0.25mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol. |
| Purity |
Greater than 90.0% as determined by SDS-PAGE. |
| Description |
MEP1B produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 642 amino acids (21-654aa) and having a molecular mass of 72.6kDa. (Molecular size on SDS-PAGE will appear at approximately 70-100kDa).MEP1Bis expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques. |
| Protein Background |
Meprin A subunit beta (MEP1B) belongs to the astacin family of zinc endopeptidases 1, 2. MEP1B is a disulfide-linked, tetrameric metalloendopeptidase in renal brush border membranes. MEP1B is highly regulated, secreted cell-surface metalloendopeptidase, which is amply expressed in the kidney and intestine. |
| Expression host |
Sf9 Insect cells. |
| Synonyms |
Meprin A subunit beta (EC:3.4.24.63), Endopeptidase-2, Meprin B, Mep1b, Mep-1b. |
| Reagent Appearance |
Sterile filtered colorless solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
LPAPEKFVKD IDGGIDQDIF DINQGLGLDL FEGDIKLEAN GKNSIIGDHK RWPHTIPYVL EDSLEMNAKG VILNAFERYR LKTCIDFKPW SGEANYISVF KGSGCWSSVG NIHAGKQELS IGTNCDRIAT VQHEFLHALG FWHEQSRADR DDYVIIVWDR IQPGKEHNFN IYNDSVSDSL NVPYDYTSVM HYSKTAFQNG TESTIVTRIS EFEDVIGQRM DFSDYDLLKL NQLYNCTSSL SFMDSCDFEL ENICGMIQSS GDSADWQRVS QVLSGPESDH SKMGQCKDSG FFMHFNTSIL NEGATAMLES RLLYPKRGFQ CLEFYLYNSG SGNDQLNIYT REYTTGQQGG VLTLQRQIKE VPIGSWQLHY VTLQVTKKFR VVFEGLRGPG TSSGGLSIDD INLSETRCPH HIWHIQNFTQ ILGGQDTSVY SPPFYSSKGY AFQIYMDLRS STNVGIYFHL ISGANDDQLQ WPCPWQQATM TLLDQNPDIR QRMFNQRSIT TDPTMTSDNG SYFWDRPSKV GVTDVFPNGT QFSRGIGYGT TVFITRERLK SREFIKGDDI YILLTVEDIS HLNSTSAVPD PVPTLAVHNA CSEVVCQNGG ICVVQDGRAE CKCPAGEDWW YMGKRCEKRG STRDVEHHHH HH. |
