MED7 Human

Data sheet

Formulation

The MED7 solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, and 10% glycerol.

Purity

Greater than 85% as determined by SDS-PAGE.

Description

MED7 Human Recombinant produced in E. coli is a single polypeptide chain containing 256 amino acids (1-233) and having a molecular mass of 29.7 kDa.MED7 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques

Protein Background

MED7 is a member of the Mediator complex subunit 7 family. The activation of gene transcription is a multistep course which is initiated by factors which identify transcriptional enhancer sites in DNA and collaborate with co-activators to direct transcrip

Expression host

E.coli.

Synonyms

Mediator complex subunit 7, cofactor required for Sp1 transcriptional activation subunit 9 33kDa, Activator-recruited cofactor 34 kDa component, Transcriptional coactivator CRSP33, RNA polymerase transcriptional regulation mediator subunit 7 homolog, medi

Reagent Appearance

Sterile Filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSMGEPQQV SALPPPPMQY IKEYTDENIQ EGLAPKPPPP IKDSYMMFGN QFQCDDLIIR PLESQGIERL HPMQFDHKKE LRKLNMSILI NFLDLLDILI RSPGSIKREE KLEDLKLLFV HVHHLINEYR PHQARETLRV MMEVQKRQRL ETAERFQKHL ERVIEMIQNC LASLPDDLPH SEAGMRVKTE PMDADDSNNC TGQNEHQREN SG