| Formulation |
The MBIP solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 0.4M Urea. |
| Purity |
Greater than 85% as determined by SDS-PAGE. |
| Description |
MBIP Human Recombinant produced in E. coli is a single polypeptide chain containing 367 amino acids (1-344) and having a molecular mass of 41.7kDa. MBIP is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
MAP3K12 Binding Inhibitory Protein 1 (MBIP) is a part of the ATAC complex which consists of histones H3 and H4 with a histone acetyltransferase activity. MBIP inhibits the MAP3K12 activity in order to induce the activation of the JNK/SAPK pathway. |
| Expression host |
Escherichia Coli. |
| Synonyms |
MAP3K12 binding inhibitory protein 1, MAPK upstream kinase-binding inhibitory protein, MUK-binding inhibitory protein, MBIP. |
| Reagent Appearance |
Sterile Filtered clear solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSMAAATEL NRPSSGDRNL ERRCRPNLSR EVLYEIFRSL HTLVGQLDLR DDVVKITIDW NKLQSLSAFQ PALLFSALEQ HILYLQPFLA KLQSPIKEEN TTAVEEIGRT EMGNKNEVND KFSIGDLQEE EKHKESDLRD VKKTQIHFDP EVVQIKAGKA EIDRRISAFI ERKQAEINEN NVREFCNVID CNQENSCART DAIFTPYPGF KSHVKVSRVV NTYGPQTRPE GIPGSGHKPN SMLRDCGNQA VEERLQNIEA HLRLQTGGPV PRDIYQRIKK LEDKILELEG ISPEYFQSVS FSGKRRKVQP PQQNYSLAEL DEKISALKQA LLRKSREAES MATHHLP. |
