| Formulation |
The LIF protein was lyophilized from 1mg/ml in PBS pH 7.4. |
| Solubility |
It is recommended to reconstitute the lyophilized LIF in sterile PBS containing 0.1% endotoxin-free recombinant HSA. |
| Purity |
Greater than 95.0% as determined by SDS-PAGE. |
| Description |
Leukemia Inhibitory Factor Human Recombinant produced in HEK cells is a glycosylated monomer, having a molecular weight of 19.9kDa due to glycosylation.The LIF is purified by proprietary chromatographic techniques. |
| Protein Background |
Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence. |
| Expression host |
HEK. |
| Synonyms |
CDF, HILDA, D-FACTOR, Differentiation- stimulating factor, Melanoma-derived LPL inhibitor, MLPLI, Emfilermin, Leukemia inhibitory factor, LIF, DIA. |
| Stability |
Lyophilized LIF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LIF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Physical Appearence |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Biological Activity |
The biological activity was determined by its ability to stimulate the proliferation of Human TF-1 cells, the ED50 is typically less than 0.1ng/ml. |
