| Formulation |
The LAYN solution contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 1mM DTT and 10% glycerol. |
| Purity |
Greater than 85% as determined by SDS-PAGE. |
| Description |
LAYN Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 237 amino acids (22-235) and having a molecular mass of 26.7kDa.LAYN is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
LAYN, also known as Layilin, contains 1 c-type lectin domain. This protein is receptor for hyaluronate and Interacts with NF2, RDX and TLN1. The C-terminal domain interacts with the N-terminal domain of RDX. |
| Expression host |
Escherichia Coli. |
| Synonyms |
Layilin |
| Reagent Appearance |
Sterile filtered colorless solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSATGRLLS ASDLDLRGGQ PVCRGGTQRP CYKVIYFHDT SRRLNFEEAK EACRRDGGQL VSIESEDEQK LIEKFIENLL PSDGDFWIGL RRREEKQSNS TACQDLYAWT DGSISQFRNW YVDEPSCGSE VCVVMYHQPS APAGIGGPYM FQWNDDRCNM KNNFICKYSD EKPAVPSREA EGEETELTTP VLPEETQEED AKKTFKESRE AALNLAY |
| Shipping Conditions |
Store unopened antibody at 4°C or lower. Under these conditions, there is no significant loss in product performance one year from the purchase date. The reconstituted antibody is stable for at least two months when stored at 4°C. For long term storage, it is recommended that aliquots of the antibody are frozen at -20°C (frost-free freezers are not recommended). Repeated freezing and thawing must be avoided. Prepare working dilutions on the day of use. |
| Product Description |
Mouse monoclonal antibody against the amino acid stretch of KDPRIAA (the amino acid residue 339 to 345) of the periplasmic maltose-binding protein (MBP) [Escherichia coli] (Accession #: AAB59056). |
| Storage buffer |
The malE gene is inserted in a pMAL vector (Maina et al., 1988) and then expressed in E. coli JM109. The purified recombinant MBP protein was then used for mouse immunization. |
| Recommendations on use |
Theammonium sulfate-precipitated ascites fluid is in PBS + 1% BSA & then lyophilized. |
| Westren Blotting |
Immunofluorescence: Typical working dilution on frozen sections 1:200. 60 minutes primary antibody incubates at 25°C. |
| Storage and Stability |
Recommended: working dilution on Western bloting 1:500. 60 minutes primary antibody incubates at 25°C. |
