KLF12 Human

Data sheet

Formulation

KLF12 protein solution (0.5mg/ml) contains 20mM Tris-HCl buffer, (pH8.0) ,10% glycerol and 0.4M Urea.

Purity

Greater than 85.0% as determined by SDS-PAGE.

Inactivation

Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 2.02 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program o

Description

KLF12 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 425 amino acids (1-402 a.a) and having a molecular mass of 46.6kDa.KLF12 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chro

Protein Background

Krueppel-like factor 12 (KLF12) belongs to the Kruppel-like zinc finger protein family and can repress expression of the AP-2 alpha gene by binding to a particular site in the AP-2 alpha gene promoter. AP-2 alpha (Activator protein-2 alpha) is a developme

Expression host

Escherichia Coli.

Synonyms

AP-2rep, AP2REP, HSPC122, Krueppel-like factor 12, Transcriptional repressor AP-2rep.

Reagent Appearance

Sterile Filtered clear solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSMNIHMKR KTIKNINTFE NRMLMLDGMP AVRVKTELLE SEQGSPNVHN YPDMEAVPLL LNNVKGEPPE DSLSVDHFQT QTEPVDLSIN KARTSPTAVS SSPVSMTASA SSPSSTSTSS SSSSRLASSP TVITSVSSAS SSSTVLTPGP LVASASGVGG QQFLHIIHPV PPSSPMNLQS NKLSHVHRIP VVVQSVPVVY TAVRSPGNVN NT