| Formulation |
KCNMB3 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.4M UREA and 10% glycerol. |
| Purity |
Greater than 85.0% as determined by SDS-PAGE. |
| Description |
KCNMB3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 149 amino acids (82-207 a.a) and having a molecular mass of 16.8kDa. KCNMB3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
The KCNMB3 belongs to a family of 4 auxiliary beta subunits found in the mammalian genome, which associate with Slo1 alpha subunits and regulate BK channel function. In humans, the KCNMB3 gene is comprised of 4 N-terminal alternative exons which produce 4 functionally distinct beta3 subunits, beta3a-d. Three variants, beta3a-c, display kinetically distinct inactivation behaviors. |
| Expression host |
Escherichia Coli. |
| Synonyms |
Potassium Channel Subfamily M Regulatory Beta Subunit 3, Potassium Large Conductance Calcium-Activated Channel, Subfamily M Beta Member 3, Calcium-Activated Potassium Channel, Subfamily M Subunit Beta-3, Charybdotoxin Receptor Subunit Beta-3, Big Potassium Channel Beta Subunit 3, Maxi K Channel Subunit Beta-3, MaxiK Channel Beta-Subunit 3, BK Channel Beta Subunit 3, BK Channel Subunit Beta-3, K(VCA)BETA-3, SLO-BETA-3, BKBETA3, Hbeta3, KCNMB2, KCNMBL, Large Conductance, Voltage And Ca2+ Activated Potassium Channel Maxi K Beta 3 Subunit, Calcium-Activated Potassium Channel Regulatory Subunit, SLOBETA3, Calcium-activated potassium channel subunit beta-3. |
| Reagent Appearance |
Sterile Filtered colorless solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGSKPFMLSI QREESTCTAI HTDIMDDWLD CAFTCGVHCH GQGKYPCLQV FVNLSHPGQK ALLHYNEEAV QINPKCFYTP KCHQDRNDLL NSALDIKEFF DHKNGTPFSC FYSPASQSED VILIKKYDQ. |
