Internalin-A

Data sheet

Formulation

Internalin-A is lyophilized from a 0.2um filtered concentrated solution in 20mM PB pH7.4 and 300mM NaCl.

Solubility

It is recommended to reconstitute the lyophilized Internalin-A in sterile 2XPBS not less than 100ug/ml, which can then be further diluted to other aqueous solutions.Important note: If the concentration of NaCl is less than 150mM, the protein solution beco

Purity

Greater than 95.0% as determined by SDS-PAGE and HPLC.

Description

Internalin Listeria monocytogenes Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 374 amino acids and having a molecular mass of 42.7kDa. The Internalin-A is fused with an 8 amino acid His tag at C-terminus.

Protein Background

Internalins are surface proteins located on Listeria monocytogenes. Internalins exist in two known forms, InlA and InlB. Internalins are used by the bacteria to invade mammalian cells via cadherins transmembrane proteins. The precise role of the Internal

Expression host

Escherichia Coli.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized Internalin-A although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Internalin-A should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recomme

Amino acid sequence

MANENNFLEI MSETKEIKIV NIPDNNLKKV LNKSLNKSEN SDLTVKDLES IEYLYGIAEN ISNIEGLEYC KNLKILSLQN NDNSKKENFN TITDLSPLKY LKNLVVLDLR NNKISDLSPL ENLTNLESLR LSGNNISNIS PLNKLESLTT LTLSYNEITD ISTISNLKNL THLALYNNKI EDISSLKENT KLQNLSLGFN KIKDISVLSN LKNLYDLSLE ENNIKSIKSL SN