IL 12 Mouse

Data sheet

Formulation

The protein was lyophilized from 0.5xPBS, pH 7.5.

Solubility

It is recommended to reconstitute the lyophilized murine IL12 in sterile distilled pyrogen free water at 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 95% as determined by SDS-PAGE.

Description

Interleukin-12 Mouse Recombinant produced in Sf9 insect cells is a glycosylated disulfide linked heterodimeric polypeptide containing 506 amino acids and having a molecular weight of 75 kDa comprised of disulfide-bonded 35 kDa (p35) and 40 kDa (p40) subun

Protein Background

IL-12 is a heterodimeric cytokine that stimulates the production of interferon gamma from T-cells and natural killer cells, and also induces differentiation of Th1 helper cells. It is an initiator of cell-mediated immunity.

Expression host

HEK-293 Cells.

Synonyms

NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, Edodekin-alpha, IL-12.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized mouse IL-12 although stable at room temperature for 3 weeks, should be stored below -18°C. Upon reconstitution IL12 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrie

Amino acid sequence

p35 Subunit: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA.p40 Subunit: MWELEKDVYV VEVDW

Biological Activity

IL-12 Mouse has full biological activity when compared to standards. The ED50 is determined by the dose-dependent induced expression of CD25 on CD8+ mouse T cells is 10-20 ng/ml corresponding to a specific activity of 50,000-100,000units/mg.

Protein concentration

Lateral flow immunoassay.

link

Purified monoclonal IgG by protein A chromatography.