HSPB2 Human

Data sheet

Reconstitution

1mg/ml in PBS (after reconstitution).

Formulation

The HSPB2 solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT and 20% glycerol.

Solubility

This antibody was produced in BALB/c mice.

Purity

Greater than 90% as determined by SDS-PAGE.

Description

HSPB2 Human Recombinant produced in E. coli is a single polypeptide chain containing 206 amino acids (1-182) and having a molecular mass of 22.8kDa.HSPB2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic technique

Protein Background

Heat shock protein beta-2 (HSPB2) is a member of the small heat shock protein (HSP20) family. HSPB2 is a regulator of DMPK kinase.

Expression host

E.coli.

Synonyms

Heat shock 27kDa protein 2, DMPK-binding protein, MKBP, HSP27, LOH11CR1K.

Reagent Appearance

Sterile Filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSHMSGRSV PHAHPATAEY EFANPSRLGE QRFGEGLLPE EILTPTLYHG YYVRPRAAPA GEGSRAGASE LRLSEGKFQA FLDVSHFTPD EVTVRTVDNL LEVSARHPQR LDRHGFVSRE FCRTYVLPAD VDPWRVRAAL SHDGILNLEA PRGGRHLDTE VNEVYISLLP APPDPEEEEE AAIVEP.

Antibody Properties

Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Titer

Antibody is shipped lyophilized at ambient temperature.

Protein content

By direct ELISA, 1:20,000 dilution will yield >1.0 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories).

Amino Acid Sequences

No cross reactivity with murine IL-8 was detected.