HPR Human

Data sheet

Reconstitution

1 mg/ml in PBS (after reconstitution).

Formulation

HPR protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol 1mM DTT and 0.15M NaCl.

Solubility

The antibody was produced in BALB/c mice.

Purity

Greater than 85% as determined by SDS-PAGE.

Description

HPR Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 352 amino acids (20-348 a.a) and having a molecular mass of 39.3kDa.HPR is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromat

Protein Background

Haptoglobin-Related Protein also known as HPR, is a primate-specific plasma protein related with apolipoprotein L-I (apoL-I)-containing high-density lipoprotein (HDL) particles which shown to be a part of the innate immune defense. HPR-bound hemoglobin mi

Expression host

Escherichia Coli.

Synonyms

Haptoglobin-Related Protein, A-259H10.2, Haptoglobin-Related Locus, HP.

Reagent Appearance

Sterile Filtered clear solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSLYSGNDV TDISDDRFPK PPEIANGYVE HLFRYQCKNY YRLRTEGDGV YTLNDKKQWI NKAVGDKLPE CEAVCGKPKN PANPVQRILG GHLDAKGSFP WQAKMVSHHN LTTGATLINE QWLLTTAKNL FLNHSENATA KDIAPTLTLY VGKKQLVEIE KVVLHPNYHQ VDIGLIKLKQ KVLVNERVMP ICLPSKNYAE VGRVGYVSGW GQ

Antibody Properties

Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Titer

Antibody is shipped lyophilized at ambient temperature.

Protein content

By direct ELISA, 1:20,000 dilution will yield >1.0 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories). 1:2,000 dilution will completely inhibit a thymocyte co-stimulation assay using suboptimal doses of concanavalin A.